This page includes videos that were taken as stills at various frame rates. These videos are hosted through my homepage on Vimeo. The rate at which these videos play back is at thirty frames per second. The actual rate of acquisition is dependent on the scale which the biological event takes place.
Videos
PhotoConversion
Acquisition of 3D images over time two channel detection. A batch of 3t3 cells that were transduced with photoconvertable dendra protein. The central cell was selectively activated using UV light. Imaris was used to complete the rendering. Imaging was performed on a point scanning confocal microscope.
Mitochondria
This video of these lively mitochondria was performed using a mix between fluorescence microscopy and differential interference microscopy (DIC). The flourescence and DIC channels were imaged simultaneously usings separate dectectors in separate light paths.
Ex-Vivo Cultures
This video was obtained on a stereo-microscope with a color camera. The culture was backlight using oblique illumination technique. This heart had been kept alive in culture for greater than 24 hours before imaging.